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KMID : 0359319960360010187
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Korean Journal of Veterinary Research
1996 Volume.36 No. 1 p.187 ~ p.193
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Comparative analyses of Theileria sergenti isolated from Korea and Japan by Southern hybridization and polymerase chain reaction
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Onuma, Misao
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Abstract
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The T sergenti DNA fragments used as probes of KTS1(2.4kb) and KTS3(1.5kb) were labeled with digoxigenin-11-dUTP for the Southern hybridization. T sergenti DNAs from different geographic locations(Korea; Chonbuk, Kwngbuk, Chungnam, Kangwon, Cheju island, Japan; Shintoku, Shintoku 9209, Shintoku 9201, Shintoku 9202, Shintoku 9102) which had been digested with Pst I and EcoR I were probed by the digoxigenin-11-dUTP-labeled KTS1 and KTS3. As the results, the samples from Chonbuk, Kyungbuk, Cheju island in Korea and Shintoku, Shintoku 9209, Shintoku 9201, Shintoku 9102 in Japan were positively reacted, but the others from the other locations not reacted.
In the comformation test of T sergenti DNA from different geographic locations, all of the samples were positively detected by PCR amplification.
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KEYWORD
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